Sains Malaysiana 53(8)(2024): 1889-1900

http://doi.org/10.17576/jsm-2024-5308-13

Induction of Polyploidy in Dalbergia latifolia Roxb. using Oryzalin

(Peinduksian Poliploidi dalam Dalbergia latifolia Roxb. menggunakan Oryzalin)

INDIRA RIASTIWI^1,2, WITJAKSONO¹, ULFAH JUNIARTI SIREGAR³ & DIAH RATNADEWI^2,*

¹Research Center for Applied Botany, Research Organization for Life and Environmental Sciences, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, 16911, West Java, Indonesia

 ²Department of Biology, Faculty of Mathematic and Natural Sciences, IPB University, Dramaga Campus, Jl. Raya Dramaga, Bogor, 16680, West Java, Indonesia

 ³Department of Silviculture, Faculty of Forestry and Environment, IPB University,

Dramaga Campus, Jl. Raya Dramaga, Bogor, 16680, Indonesia

Diserahkan: 4 Mac 2024/Diterima: 17 Jun 2024

Abstract

The demand for rosewood wood keeps increasing, while the availability of large-diameter rosewood trees is becoming scarce. Rosewood is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN) and listed in Appendix II of Convention of International Trade of Endangered Species (CITES). The objective of this research was to develop a method to obtain fast-growing and more vigorous rosewood seedlings for quicker harvest through polyploidy induction using oryzalin. This experiment successfully produced tetraploid rosewood through polyploidy induction from seedlings. Polyploidy induction was carried out by soaking rosewood germinated seeds for 12 and 24 h in oryzalin solutions at concentrations of 0, 3.75, 7.5, 15, 30, 45, and 120 µM. The germinated seeds were then planted in a growth medium and maintained for 18 months. Ploidy level identification of those seedlings was conducted using flow cytometry and confirmed with stomata characterizations. Two accessions of tetraploid rosewood were obtained from the treatment of 15 µM oryzalin for 12 and 24 h soaking, while seven mixoploids were obtained by treatment of 12 h at various oryzalin concentrations. Induced tetraploid seedlings had longer stomata but lower stomata density and thicker leaves than their diploid counterparts. Both induced tetraploid and mixoploid rosewood seedlings did not exhibit greater vegetative vigor than their diploids up to 18 months of age. Therefore, oryzalin induced polyploidy, mixoploid and tetraploid, but it did not produce faster growing seedlings or better habitus of rosewood compared to their diploids, at least up to 18 months.

Keywords: Mixoploid; polyploidy; rosewood; seedling; tetraploid; vulnerable plant

Abstract

Permintaan terhadap kayu rosewood terus meningkat, sementara ketersediaan pokok rosewood berdiameter besar semakin berkurangan. Rosewood dikelaskan sebagai rentan oleh Kesatuan Antarabangsa untuk Pemuliharaan Alam Semula Jadi dan Sumber Daya (IUCN) dan disenaraikan dalam Lampiran II Konvensyen Perdagangan Antarabangsa Spesies Terancam (CITES). Oleh itu, perdagangannya dihadkan oleh kuota. Justeru, campur tangan teknologi diperlukan untuk menghasilkan anak benih rosewood yang cepat tumbuh untuk penuaian yang lebih cepat. Objektif penyelidikan ini adalah untuk membangunkan dan mendapatkan anak benih rosewood yang cepat tumbuh dan lebih vigor melalui induksi poliploidi menggunakan oryzalin. Uji kaji ini berjaya menghasilkan tetraploid rosewood melalui induksi poliploidi daripada anak benih. Induksi poliploidi dilakukan dengan merendam anak benih rosewood selama 12 dan 24 jam dalam larutan oryzalin pada kepekatan 0, 3.75, 7.5, 15, 30, 45 dan 120 µM. Anak benih kemudian ditanam dalam medium pertumbuhan dan dipelihara selama 18 bulan. Pengenalpastian tahap ploidi anak benih dilakukan menggunakan sitometri aliran dan disahkan dengan pencirian stomata. Dua penerimaan tetraploid rosewood diperoleh daripada rawatan oryzalin 15 µM selama 12 dan 24 jam perendaman, manakala tujuh miksoploid diperoleh daripada rawatan 12 jam pada pelbagai kepekatan oryzalin. Anak benih tetraploid yang diinduksi mempunyai stomata yang lebih panjang tetapi kepadatan stomata yang lebih rendah dan daun yang lebih tebal berbanding anak benih diploid. Kedua-dua anak benih tetraploid dan miksoploid yang diinduksi tidak menunjukkan daya vegetatif yang lebih besar berbanding rakan diploid mereka sehingga umur 18 bulan. Oleh itu, poliploidi berhasil diinduksi oleh oryzalin, sama ada mixoploid dan tetraploid, tetapi tidak menghasilkan anak benih rosewood yang tumbuh lebih cepat atau lebih baik berbanding rakan diploidnya, sekurang-kurangnya sehingga usia 18 bulan.

Kata kunci: Anak benih; miksoploid; poliploidi; rosewood; tetraploid; tumbuhan rentan

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*Pengarang untuk surat-menyurat; email: dratnadewi@apps.ipb.ac.id